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The expression of a selection of nine genes from the microarray analyses was validated using reverse-transcription quantitative PCR (RT-qPCR).
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The hierarchical clustering analyses were validated using c1Valid R Package [ 32], by internal and stability cluster validation measures.
The results from the SDOF analyses were validated using the commercial finite element (FE) program ABAQUS.
DCE analyses were validated using Stata 14.1.
The analyses were validated using Statistica 7.0 (StatSoft Inc., Tulsa, OK, USA).
The ChIP analyses were validated using single ChIP-qPCR with different antibodies for 11 42 randomly selected peaks (Supplementary Table S5).
The screening method was validated using transgenic barley grain analysed during development and subjected to germination.
Large rearrangement analysis was validated using NGS, microarray comparative genomic hybridization (CGH), and multiplex ligation-dependent probe amplification analyses (MLPA).
The analytical estimations are compared to the results of the finite element analyses, which in turn are validated using available test results.
In another study, currently under submission, the MANQ has been validated using various analyses (such as factor analyses, Cronbach Alpha, test-retest and interrater reliability testing, and corresponding values on the MANQ for each category of the CAN).
Microarray analyses were used to investigate changes in gene expression associated with changes in starch levels and were validated using qPCR analyses of expression of selected genes.
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