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The expression of a selection of nine genes from the microarray analyses was validated using reverse-transcription quantitative PCR (RT-qPCR).
The prespecified cutoff point for CD8+ iTIL (0 vs. ≥1) used for the analyses was validated in our previous studies [ 16, 35].
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The hierarchical clustering analyses were validated using c1Valid R Package [ 32], by internal and stability cluster validation measures.
The theoretical analyses are validated by finite element analyses.
The analyses are validated by both simulation and measurement results.
The analyses are validated experimentally on a prototype machine.
Subsequently, microarray analyses were validated by RT-PCR and qRT-PCR.
Interpretive analyses were validated with a collaborative research team and the participants themselves.
Laser diffraction analyses were validated against sieve-type measurements for the same material.
The results from the SDOF analyses were validated using the commercial finite element (FE) program ABAQUS.
However, it might take a while before advanced analyses are validated and available.
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