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Double-immunocytochemical analyses was performed utilizing the Tyramide Signal Amplification™-Direct (Red) system (NEN Life Sciences, Boston, MA).
Double-immunocytochemical analyses was performed utilizing the Tyramide Signal Amplification™-Direct (Red) system (NEN Life Sciences, Boston, MA) to detect alpha-syn.
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It must be emphasized here that both AFLP and RFLP analyses were performed utilizing non-methylation and methylation sensitive restriction enzymes, thereby uncovering differences which cannot be attributable solely to sequence modifications.
Spectral analyses were performed utilizing the XWIN-NMR BRUKER suite.
One-dimensional Western blot analyses were performed utilizing standard procedures.
Statistical analyses were performed utilizing the Statistical Analysis System SASS, version 8.02) [ 29].
All analyses were performed utilizing the SAS 9.4 software [ 36].
Western blot analyses were performed utilizing standard procedures.
All statistical analyses were performed utilizing Statistical Package for the Social Sciences 17.0 (SPSS Inc., Chicago, IL).
All analyses were performed utilizing the SPSS software package (Version 11.5, SPSS Inc., Chicago, IL).
The spectra were generated on WCX2 chips and protein peaks clustering and classification analyses were performed utilizing Biomarker Wizard and Biomarker Patterns software packages, respectively.
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