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Statistical significance for these analyses was determined using Fisher's exact test.
Statistical significance for these analyses was determined using chi-square tests or Fisher's exact tests (if there were less than five individuals for any allele or genotype).
The inbreeding coefficient is a measure of deviations from expected heterozygosity under random mating and ranges from −1 to 1. Statistical significance for these analyses was determined using Fishers Exact tests.
The evolutionary model used for the phylogenetic analyses was determined using ModelTest v3.0 with Akaike information criterion (AIC) [ 66].
The correlation between expression profiles of selected genes obtained from real-time PCR and RNA-seq data analyses was determined using MS Excel.
The significance of the regression analyses was determined using a one-tailed t-test in the positive direction, in order to test the hypothesis that there is a positive relationship between estimated selection pressure on MCPH1 and the phenotypic variables as in [ 25].
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The nucleotide sequences of the cry8Ka1 variant genes encoding toxins active against A. grandis larvae (according to bioassay analyses) were determined using a 3130xL Genetic Analyser (Applied Biosystems).
Elemental analyses were determined using analyzer Costech instruments elemental combustion system 4100.
Differences during ERG analyses were determined using ANOVA statistical analysis.
The percentages of activated cells and CFSE analyses were determined using FlowJo software (Treestar, Ashland, OR).
The estimated sample sizes for all likelihoods, trees and model parameters from both analyses were determined using Tracer to ensure that the MCMC procedure was effectively sampling from the posterior distribution.
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