Exact(1)
Integration of the data by Procrustes and Co-inertia analyses was achieved using the functions "procrustes" and "cia" of "Vegan" [ 44] and "Made4" [ 48] packages, respectively.
Similar(59)
Quantitative analyses were achieved using an AX10 Zeiss Apotome (Zeiss Microscopy).
Phylogenetic analyses were achieved using the methods of Neighbor-Joining (BioNJ) [ 43] implemented in SeaView [ 44], or run under a WAG model with estimated gamma distribution parameter for the Maximum Likelihood (PhyML) [ 45], both of them with 1000 bootstrap iterations.
Genes were clustered based on expression profiles determined through the use of a post-hoc chi-square test statistic in pair-wise analyses and was achieved using custom Perl scripts, which are available upon request [ 26].
Four genes were sufficient for accurate class assignment and importantly, this was achieved using linear discriminate analyses, a gold standard approach to tasks of this kind.
This was achieved using the following procedure.
Normalization was achieved using SNORD47.
For the data stored in the workset, a succession of analyses can be achieved using a number of implemented comparative genomics and molecular evolution analysis tools.
For all analyses, however, syntheses were achieved using a weighted linear combination of the study estimates so as to give more weight to studies of larger size.
Negative ion mode analyses of polar metabolites were achieved using a HILIC method under basic conditions.
As only a limited number of cells can be irradiated per day, typically <25 000, most of the analyses on cellular signalling can only be achieved using semi-quantitative immunostaining methods in this study.
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