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The research design for this study was evaluated and ethically approved by an independent medical ethical committee (METiGG, Utrecht, The Netherlands).> We conducted power analyses using the software Quanto [ 50].
Using 384 SNPs from Illumina BeadXpress system, the 380 genotypes were reduced to 285 genotypes after genetic diversity and population structure analyses using the software packages Powermarker (Liu and Muse 2005), and Structure 2.2 (Pritchard et al. 2000), respectively.
When applied to pedigree data only, the proposed method still may have improved power over traditional TDT-based methods, as shown by analyses using the software FBAT.
We perform global sensitivity analyses using the software package SBML-SAT [ 41].
Analyses using the software AWTY showed convergence of all MCMC runs.
X-ray images were analyzed by reconstructed 3D quantitative analyses using the software VGstudio MAX 2.0.
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SEM analysis was performed using Zeiss EVO50, U.K. and photographs were analysed using the software provided by Zeiss EVO50.
Sequences were analysed using the software Genalys obtained at http://software.cng.fr/.fr/
We conducted meta-analyses using the software package Comprehensive Meta Analysis version 2 (Biostat, Inc. 2007).
The data was analysed using the software package STATA IC10.
The chromatograms were analysed using the software Lab Solutions (Shimadzu).
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