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The envelope of the results of two pushover analyses using the constructed load patterns is considered as the seismic demands of the building structure.
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We then confirmed that the large difference in IRES dependent-eGFP expression detected by FACS and Western analyses using the pMigR constructs remains true with the pPRIG vector.
The amino acid sequences of invertebrates and vertebrates were aligned using ClustalW v2 software [ 49] and neighbor-joining trees with bootstrap values were constructed for phylogenetic analyses using the MEGA v6 software [ 50].
Parasites transfected with the replacement construct b3D-LplA1-REP were analysed using the gene locus specific primer set Pb-3/Pb-11 (amplifying a 1837 bp product) or Pb-10/Pb-12 Pb-10/Pb-12 Pb-10/Pb-12roduct).
WW developed earlier the principle of these analyses [ 34, 84, 121, 135], prepared phylogenetic analyses using published alignments, constructed the split networks, drafted the manuscript and discussed the results.
Data were analysed using the structural equation modelling to examine the relationships among the constructs.
The phylogeny indicated that DmCatD has features which suggest its distancing from "classical" cathepsins D. Bioinformatic analyses using a chimeric construct were employed to predict post-translational modifications.
As a prelude to future functional analyses using RNAi suppression, constructs containing the sequences of interest were introduced into F. vesca 'Hawaii 4' using Agrobacterium-mediated transformation, as described below.
Analyses using eBURST and trees constructed in MEGA4 resolved isolates into clonal complexes or clusters.
Tree diagrams constructed by cluster analyses, using eight length factors in a given GPCR, revealed possible length relations among GPCRs and defined at least three groups.
Future studies to explore these targets could consider analyses using gene-specific 3-UTR luciferase constructs in which putative miRNA binding sites have been mutated, as well as an evaluation of their effects in the specific target cells of interest, such as hepatoblasts.
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