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In further analyses, the expression of various chemokines and chemokine receptors controlling the migration of DC subsets was investigated.
In order to perform correct flow cytometry analyses, the expression of both receptors was evaluated within gated CD3 positive cell population in all groups.
From the results obtained in these experiments, we carried out further investigations using WNT-signaling pathway PCR array, which analyses the expression of a focused panel of genes related to the different pathways regulated by WNT.
Based on in-silico analyses, the expression of PR-1b and PR-1c is restricted to flowers and fruits, while PR-1a transcripts are present in many different tissue types.
To confirm the results of RNA-Seq analyses, the expression of 27 whitefly genes, including ten HTG candidates, was measured by qRT-PCR with three biological RNA samples of isolated bacteriocytes and whole-body samples of adult whiteflies.
As revealed by microarray and Western blot analyses, the expression of GS is significantly lower in depressed patients, further corroborating the fundamental importance of astrocytes to the disorder (Choudary et al., 2005; Miguel-Hidalgo et al., 2010).
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According to RT-PCR analyses, the expressions of several key flowering-related genes were down-regulated.
To assess if cHes6-1 promotes neuronal differentiation, we ectopically expressed this gene in the embryonic chick neuroepithelium and analysed the expression of the early neuronal marker cDelta1 [37].
We next analysed the expression of a number of adipocyte markers and regulatory factors that were expressed during mouse adipogenesis.
Furthermore, we analysed the expression of genes related to stomatal development by qRT-PCR.
A confocal microscope (LSM510; Carl Zeiss; Germany) was used to analyse the expression of the target protein [24].
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