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In all the correlation analyses, the expression level values were transformed to logarithmic scale (log2), and the significance levels were calculated using Bonferroni correction.
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It was also analysed the expression level of N-cadherin and vimentin mesenchymal markers, which are frequently expressed in carcinoma cells that have undergone EMT.
To determine the rationale for these differences in expression patterns, we analysed the expression level of all the co-amplified genes in five xenografted and one fresh human gliomas containing dmins, the sequence of which has been previously analysed in detail [14], [15].
To analyse the expression level as a qualitative variable, the patients were subdivided into equal groups around the median.
The experimentally normalised d Ct values were converted to 39 – Ct used to analyse the expression level of human mRNA transcripts.
The aim of this study was to develop a reliable and sensitive real-time quantitative PCR assay to analyse the expression level of each isoform.
Therefore, we analysed the expression level of apple MLO genes identified in this study in response to the interaction with P. leucotricha.
On the GeneChip Mouse Genome 430 2.0 Array, over 45,000 probe sets analyse the expression level of over 39000 transcripts and variants from over 34000 well characterized mouse genes.
In order to analyse the expression level of each mRNA, real-time quantitative PCR was performed using the ABI StepOne Realtime PCR system (Applied Biosystems, Foster City, CA, USA).
DNA microarray technologies are used to analyse the expression level of many genes in a single reaction quickly and in an efficient manner.
We analysed the expression level of EIF5A2 in tumours with different thickness and ulceration, as thickness and ulceration are well-known prognostic markers for patients with PM.
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