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In the following parametric analyses, parameters identical with the base-case ones are used unless otherwise specified.
However, when performing tracer kinetic analyses, parameters representing innervation (i.e. K i, VT, VS and BPND) can be obtained together with parameters representing flow and extraction (i.e. K1).
We set the analyses parameters to 100 runs and 20 million iterations.
We used PHYML version 2.4.4 [60] for maximum likelihood analyses, MrBayes version 3.1.2 [61] for Bayesian analyses (parameters: 2 million generations, trees sampled every 100 cycles, datasets partitioned by gene where appropriate, 50% burn in, two runs of four chains heated to 0.2), and PAUP* version 4.0 b10 [62] for parsimony analyses (full heuristic with 100 random repetitions).
In univariate sensitivity analyses, parameters were varied by ±50%.
In all analyses, parameters with non-normal distributions were used after log-transformation.
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For these analyses, parameter values were ranked and binned in order to smooth the signal and reduce the background noise.
Before the main analyses, parameter priors were determined based on MCMC analyses following the approach of Schultz and Churchill [ 77] in SIMMAP.
From all analysed parameters only surgery time classification proved to have a significant relationship with VTE.
In order to analyse parameters that may influence the abovementioned standard preparation technique, the synthesis was carried out with three modifications for test purposes.
Descriptive statistics were used to analyse parameters.
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