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After the ribosomal RNA removal, the analyses of the wheat roots transcriptome profiling involved 3 main-steps: 1) sequential mapping of reads to different reference datasets (UniGene-EST of T. aestivum, MicroRNAs-databank of T. aestivum and A. brasilense genome sequence); 2) de novo assembly of unmapped reads; and 3) mapping of unmapped reads to de novo assembled ESTs.
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Multi-element analyses of the untreated wheat straw and fiber fractions were conducted by inductively coupled plasma-optical emission spectroscopy (Optima 5300 DV, PerkinElmer, Waltham, MA, USA).
Various genetic approaches have been used to understand genetic control of −P tolerance in wheat; these include aneuploid analyses of the nulli-tetrasomic series and wheat alien chromosome addition lines of the cultivar 'Chinese Spring' (CS) and quantitative trait locus (QTL) mapping [ 3- 6].
Our qPCR expression analyses of seven wheat genes associated with the suppression of fungal virulence factors have demonstrated similar FHB-responsive inductions in the cultivars Dream and Sumai 3.
Sequencing analyses of the fragments amplified from both wheat and pathogen for PST_c40 and PST_c88 confirmed that the two TDFs were of wheat origin.
To analyse the differential UPF profiles of the wheat samples a hierarchical clustering (Pearson's correlation, average linkage) was carried out.
Recent structural analyses of the ES6 elements of yeast, wheat and mouse 18S rRNAs showed that the conserved rm1 and rm2 sequences are located in a stem-loop structure (Alkemar and Nygard, 2006).
(a) Unprocessed rice husks and structures generated from rice husks by 2,600 consecutive laser pulses with pulse energies of (b) 0.19, (c) 0.38, and (d) 0.58 mJ. Figure 6 EDS analyses of unprocessed wheat straws and synthesized structures.
By identifying similar sequences, the clusters identified here could be compared with those reported in other transcriptome analyses of developing wheat grain.
The ten clusters identified here were compared with those reported following other transcriptome analyses of developing wheat grain [ 5, 33] (see Additional file 3).
The clusters identified here can be compared with those reported in other transcriptome analyses of developing wheat [ 4, 8] and barley [ 6] grain by identifying similar sequences [see Additional file 2].
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