The precision of the method, checked by repetitive analyses of the same sera, showed an average coefficient of variation of 5.7%.
The combined analyses of the established RA sera resulted in a prevalence of 50percentt for the autoantibodies to peptide FN-Cit1035,1036 (Table 2), LTVGLTXXGQPRQY (X represents citrulline), in CCP2-positive established RA sera (n = 82).
Therefore, although potential effects of these complications and medications on presurgical and postsurgical proteomes cannot be ruled out, we speculate that the results obtained from analyses of the 19 CAS patients' sera are typical results for sera of the majority of CAS patients with aortic valve replacement surgery.
Except for albumin, Ig family members and reversed sequences in FDR Database, 109 proteins with differential levels (postsurgical versus presurgical) were identified as proteins that appear at least once with an unused ProtScore of ≧2 (99% confidence) by analyses of sera from the five patients, of which 51 proteins shared among the five patients were detected.
Although there was no mention of anti-PEG antibodies in that report, subsequent analyses of stored sera from some of the patients in that study provided clear evidence of anti-PEG antibodies that were detected both by serological techniques and by flow cytometry.
Metabolomic analyses of the livers (n=7 8/group) and sera (n=6/group) were performed by untargeted GC-MS analysis of polar metabolites, targeted GC-MS analyses of organic and fatty acids and LC-MS quantitation of amine metabolites.
In the same study, the authors established the principle of secretion of tumour-associated miRNAs into the circulation by analysing the sera of prostate cancer xenograft bearing mice.
Assays based on recombinant protein expression potentially allow direct analyses of immune sera.
Pertinently, anti-NPM antibodies in male WB mice were consistently associated with aCL, and both antibody populations appeared concomitantly, as shown by sequential analyses of WB sera.
