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For the analyses of the sequencing data, we assessed intra-population variability using the following estimators of the θ parameter based on the infinite-site-model of mutations: π, the per-site mean number of pair-wise differences between sequences [66], and by θw, based on the number of segregating sites [67].
All analyses of the sequencing data were performed with the GS FLX Software v2.0.01 (454 Life Sciences, Roche).
All processing and analyses of the sequencing data was performed with the GS-FLX Software v2.0.01 (454 Life Sciences, Roche).
All processing and analyses of the sequencing data was performed with GS-FLX Software v2.0.01 (454 Life Sciences, Roche, Germany).
Quantitative analyses of the sequencing reads revealed 6%to9%9% of genes are differentially regulated between the two phases.
The number of reads which passed the initial quality filter varied from 68.8 to ca 81.6 million and the quality analyses of the sequencing runs showed that the sequencing step was successful (data not shown).
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Analyses of the sequences were performed using Uparse software (Uparse v7.0.1001) (Edgar 2013).
Combined analyses of the sequence of cyclonic events, major mass wasting events and aerial photography interpretation over the last 40 years led to the proposal of a functional model of river system responses to these events.
More recently, phylogenetic analyses of the sequences of their ribosomal large subunit genes have concluded that Panellus stipticus is closely related to the poroid mushroom Dictyopanus pusillus.
Analyses of the sequence data were performed using DnaSP v4.10 [68].
Our analyses of the sequence reads generated from the libraries was relatively stringent with the requirement of reads having to be present at least twice to be included.
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