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We subsequently focused on quantitative image analyses of the nucleus.
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Analyses of the nuclei of Hoechst-33258-stained ookinetes by fluorescence microscopy showed a strong fluorescence signal similar to that of wild-type ookinetes (Fig. 6A).
Moreover, meta analyses of the caudate nucleus network and other disorders showed similarities for these disorders with HD, in particular with a high enrichment for inflammatory pathway genes.
Ano3 seems to play an important role in the mammalian brain since it was found to be a hub gene in weighted gene co-expression analyses of the caudate nucleus (Oldham et al., 2006), whereas it was also identified as a hub gene in a module of co-expressed genes that had low expression in the embryonic cortex and hippocampus in humans, but progressively increased with age (Kang et al., 2011).
Only in the lower brainstem, enhanced α-synuclein immunoreactivity within neurons in somatodendritic distribution and throughout the neuropil was observed in independent analyses of the dorsal motor nucleus of the vagus (Fig. S7).
Our analyses of the positions of nuclei demonstrate that the rate of expansion is consistent between muscle cells along the entire length of the tail during tail extension.
We analysed this process in activated Δfact-lgam male gametocytes by FACS analyses of the DNA content of the nuclei of activated males and fluorescence microscopy of the nuclei of activated males after staining with the DNA-specific dye Hoechst-33258.
We investigated cell cycle dynamics using flow cytometric analyses of the DNA content of the nuclei of MCF-7 breast cancer cells.
Cellular imaging and genomic analyses have revealed substantial compartmentalization of the nucleus, and have suggested that this compartmentalization functions in gene regulation [ 17- 19].
Cell migration was analysed by marking the position of the nucleus in individual cells in each frame to obtain migration tracks.
In order to determine the kinetic properties of U1-70K we performed FRAP analyses on speckles and nucleoplasmic regions of the nucleus.
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