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DAR provided sequence data for CR1 analyses of the library and made arrangements associated with acquisition of crocodile blood.
In order to perform experimental analyses of the library, first a potential target site preference of the transposon was tested.
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Specifics of 16S rRNA gene amplification, cloning, sequencing, and analyses of the libraries are provided elsewhere.[52] A few chi-meric sequences were found via dual tree comparisons and not included in the final phylogenetic analyses.
Since the systematic biases present in the control library would influence the ChIP-enriched libraries as well, it is not inconceivable that more thorough analyses of the control library could potentially reduce false positive rate and false negative rate in binding sites identification, while concurrently provide insights into the underlying chromatin structure.
A high degree of reproducibility of the sequence data generated from independent analyses of the same library is essential for accurate detection of subtle changes in gene expression.
DP coordinated the construction, sequencing and analyses of the EST library.
Phenotypic analyses of these libraries revealed the existence of three genes, Regulator of Awn Elongation 1 (RAE2), Rand, and RAE3, involved in the loss of long awns in cultivated rice.
BLAST analyses of the cDNA libraries sequences were performed on the NCBI server.
Quantitative real-time RT-PCR (qRT-PCR) analyses of the cDNA libraries confirmed cDNA molecules that are associated with different developmental stages (e. g. "on year" leaves in July, "off year" leaves in July, leaves in November) and fruits.
Different subtraction mRNA libraries were constructed and analyses of these libraries revealed numerous mosquito genes that are up and down regulated by infection.
Analyses of these libraries for differential gene expression revealed over 200 differentially expressed genes, including up-regulation of melanogenesis-associated genes tetrahydroxynaphthalene reductase (344-fold increase) and scytalone dehydratase (231-fold increase), and additional genes located in a 48.8 kilobase melanin biosynthetic gene cluster of strain VdLs.17.
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