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Regression analyses of the Ct values generated by the log10 dilution series gave R2 values for all reactions in excess of 0.99 (Table 1).
Clinical analyses of the CT data showed the location and degradation of the implants in the subarachnoid space in pigs and dogs.
However, several studies suggest that the amount of alveolar flooding, observed during morphological analyses of the CT scan images, exhibits an inverse correlation with PaO2 dynamics [ 28, 37, 38].
For this reason, in this study we used logistic regression analysis to eliminate any possible bias, and separate, independent analyses of the CT data were also conducted by two investigators who did not know the experimental group to which the patients belonged.
To evaluate whether each pair of primers conform to the efficiencies within the range 0.9-1.1 0.9-1.1efficiencies were examined by estheirshing standard curvefficienciesregression analyses of the Ct (Cycle threshold) value versus the log value of 10-times dilution of each target gene (Copies) for each pair of primers (see additional file 2).
Sixteen pairs of primers representing the different subgroups of OR genes in the phylogenetic tree and one pair of β-actin primers as a reference were designed as shown in additional file 2. To assess the efficiency of amplification, standard curves were constructed using regression analyses of the Ct value versus the log value of 10-times dilution of each target gene for each pair of primers.
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When analysing the results of the CT synthesis, we note that the errors are mostly located in the sinus area and at the bone/dura/CSF boundaries (Fig. 2), regions with a low tissue discriminative power.
In addition to the effect studies, we will also gather and analyse data to realize additional outcomes: Process evaluations will be executed to assess levels of personalization and appreciation and to analyse which elements of the CT programme were most attractive and successful.
Analyses of the resulting micro-CT datasets were performed using the aforementioned software packages.
To this point, our analyses used the Ct means of all target genes on the array from each sample for examining the relationship to the reference genes.
Similarly, the opportunity to perform pooled analyses of chest CT phenotypes was seen as a major strength, as long as the technical challenges of different CT scanning and analytical protocols can be overcome.
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