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Phylogenetic analyses of the alignment were conducted using Protpar on the Phylip package [ 33] through the PIE Phylogeny Interface Environmentt) suit at.
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The recombination analyses on the alignment of the concatenated MLST loci did not reveal any recombination events.
Maximum parsimony analyses of the alignments were conducted using PAUP 4.0b10 [ 98].
The models selected for partitioned analyses of the alignments are given in supplementary table S3, Supplementary Material online.
The results shown in Figures 4, 5, and 6 have been obtained from our analyses of the alignments obtained from simulations done on the balanced (symmetric) model tree.
A database containing both the AW and NW ESTs along with the Cap3 consensus sequences (included to facilitate subsequent analyses of the alignments) was assembled.
The tree inferred with PhyML under the best model from the PCMA alignment is shown in Fig. 2. The majority-rule consensus including compatible groupings of the likelihood trees obtained from each of the three complete alignments, respectively, is shown in Fig. 3 together with bootstrap values obtained by separate likelihood bootstrap analyses of the alignments.
For the 5'-part of the CR the primer binding was more efficient, and this fragment (CR1: 440 bp) was therefore chosen for the population analyses (length of the alignment 400 bp).
The result of the phylogenetic analyses of the concatenated alignment of four genes is shown in Figure 2.
Phylogenetic analyses of the CLTC alignment, conducted to provide an estimate of the CLTC gene tree, used RAxML 7.0.4 [ 37].
Bayesian analyses of the concatenated alignment were performed with PhyloBayes MPI v1.4 (Lartillot et al. 2013) using the LG model with four gamma categories modeling the relative substitution rates across sites.
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