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Our understanding of fungal phylogeny also has advanced dramatically over the past 20 years by analyses of multiple DNA loci and increased taxon sampling.
The monophyly of subfamily Thalictroideae has been confirmed based on phylogenetic analyses of multiple DNA markers: rbcL, matK, trnL-F spacer, and 26S nrDNA [ 23], 26S nrDNA [ 24], and atpB, rbcL, and 18S nrDNA [ 39].
The relationships of these nine species have been established by phylogenetic analyses of multiple DNA sequences (multilocus sequence analysis, MLSA), which has provided a well supported tree spanning closely related Neurospora species and representatives of their neighboring genera [ 26- 28].
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Increased sequencing depth per specimen allowed for the generation of multiple DNA sequences per specimen.
Bianco, V., Scialdone, A. & Nicodemi, M. Colocalization of multiple DNA loci: a physical mechanism.
Thus, Model 2 predicted two terminators as transcription termination factors, which were validated in analyses of multiple alignment of the corresponding DNA regions.
This study demonstrates the great potential of comparative DNA sequence analyses of multiple populations from different geographical origins for understanding patterns of genetic and morphological variation at the species/population level within a biogeographical and taxonomic context.
Analyses of multiple alignments validated the presence of cross-hairpins in DNA, which were predicted in our studies by modeling the polymerase competition.
In most genome projects, DNA from only one individual was sequenced, and systematic genome-wide analyses of multiple individuals were rarely performed.
WGA amplifies nanogram levels of genomic DNA from a single blastocyst to microgram levels, which enables multiple analyses of genomic DNA, as previously noted by Akasaka et al. [ 13].
It has been recognized that comparative analyses of noncoding DNA sequences in multiple species, known as phylogenetic footprinting, can help identify conserved putative regulatory elements [ 18].
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