To compare the ability of the FGF-1 IRES to drive long term expression against the EMCV and FGF-2 IRESs, we performed analyses of expression kinetics using bicistronic vectors that express the bioluminescent renilla and firefly luciferase reporter genes.
Bioinformatic analyses of expression control sequences in promoters of co-expressed or functionally related genes enable the discovery of common regulatory sequence motifs that might be involved in co-ordinated gene expression.
It is also likely that Hmgpi is expressed in iPS cells, based on in silico analyses of expression profiles [NCBI GEO database, e.g. GSE10806 (35)].
Detailed analyses of expression patterns of miRNAs has demonstrated that a significant number of miRNAs are expressed in highly selective spatial and temporal patterns [7], [8].
Family-level analyses of expression may help to quickly identify candidate targets that are deserving of more detailed study.
In our analyses of expression noise and expression consistency, we included nine additional genomic features, which could affect the observed expression noise of a gene.
To account for this effect, we included mean expression level and gene length as explanatory variables in our statistical analyses of expression consistency.
We then investigated HOPX expression in an ATII-ATI trans-differentiation culture model in vitro, in bleomycin-induced mouse model of pulmonary fibrosis in vivo, and by database analyses of expression profiles of IPF lungs in silico.
This determination of a stable set of reference genes in this culture system will facilitate analyses of expression levels for genes of interest.
In rice, the results of analyses of expression pattern and ectopic overexpressor lines also support this viewpoint, and the evidences implicate rice WRKY proteins in transcriptional reprogramming during biotic or abiotic stresses, senescence, sugar metabolites, and morphological architecture.
(B) Comparative analyses of expression levels of fadBA, a major member of beta-oxidation system and fadM, an auxiliary player of fad system in the fadR strain K113 relative to those of the wild-type ymel.
