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However, additional NMR and biochemical analyses of a minimal element of this system (a monomeric bridge-DAD construct bound to DID) suggest that the autoinhibited structure may allow actin monomers to bind at least one of the two actin-binding sites on the FH2 domain.
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Our findings therefore suggest that carefully conducted SEM analyses with a minimal number of weak assumptions provide an important supplement to standard regression results, especially when involving imprecise assessment of multiple exposures.
This result is also consistent with our theoretical analyses of the minimal network that showed the presence of large stochastic fluctuations under a weak stimulus.
This work illustrates the rich resource of eukaryotic genome projects for data pertinent to diverse host microbial interactions, and also demonstrates that highly divergent, poorly known microbial species can be characterized via in-depth mining and phylogenomic analyses of even minimal genetic information captured from these broad-scale eukaryotic-sequencing efforts.
To learn more about the nature of the putative UAS we set out to analyse its function in the context of a minimal heterologous promoter.
DNA fragments encompassing the Su(H) bound regions were therefore placed upstream of a minimal GFP reporter and the resulting expression patterns analysed in transgenic flies.
Now the crystal structures of a minimal CDC7 DBF4 complex, with bound nucleotide or inhibitor, along with functional analyses reveal that DBF4 wraps around CDC7, stabilizing the αC helix in the N lobe and tethering the C lobe of the kinase.
These analyses clearly demonstrate that selectivity towards the products of multi-copy loci is an emergent property of a minimal RNAi system.
Subgroup analyses of patients with minimal joint space ≤/>2 mm suggested that the observed deviations were more pronounced in patients with greater radiographic severity.
Example of a minimal makefile: TARGET= thetarget.
Instead of a minimal gene set, we computationally explored a minimal cellular-function set.
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