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These two SNPs (rs9804659, rs2815752) were followed-up for replication association analyses in the sample of 365 independent case-parents obesity trios.
We performed these analyses in the sample as a whole and then, as a sensitivity analysis, repeated them restricting the analysis to Caucasians.
As the genetic variant is used as a proxy for continuing to smoke during pregnancy, we also performed similar observational analyses, in the sample of pre-pregnancy smokers of European ancestry with rs1051730 genotype.
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To assess the impact of such factors, we conducted a series of validation analyses in the largest sample (sample 2, n = 102).
Intraobserver variability was analysed in the sample of 18 nurses using the Cohen kappa, and agreement based on the value of κ was categorised as described by Altman.
For the six countries analysed in the sample, there is a large, statistically significant, and positive correlation between measles vaccination and father having secondary or higher levels of education, within each level of maternal education.
When examining the reliability of the HBICA, we conducted analyses in the full sample (i.e., sample 1).
The effect estimates of the pooled analysis were of the same direction, as compared to the analyses in the Discovery sample alone and in the Replication sample alone.
The results of the cross-sectional analyses in the original sample were confirmed by analyses of the validation sample (Table 2) (Supplementary Table 2).
Minimal processing of the fruits was performed 24 h after the heat treatment and soluble solid content, colour and texture properties were analysed in the samples during the whole storage period (0 10 days).
Progesterone was analysed in the samples before sacrifice of each animal, and in the daily samples from the intact ewes from the day of sponge withdrawal onward.
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