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Impacts remained constant after leks that switched disturbance categories between 2003 and 2007 were removed from analyses (File S1).
(XLSX 46 kb) Additional file 2: Supplemental Files: xml files for Bayesian analyses (File 1: concatenated loci; File 2: divergence dating).
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To understand root hair patterning in A. alpina, we analysed file-specific root hair production in two regions of the differentiation zone to determine the temporal spatial development of the root hair pattern.
Occasionally, we managed to maintain short-term organotypic spheroid cultures [ 43] and these were used for genetic analyses (Additional file 1 and Additional file 2: Figure S1).
The gene ontology and MapMan analyses (Additional File 3 and Additional File 4e) of the transcriptional profile indicate that the PAH response shares commonality with biotic stress responses.
To fully utilize this resource, we have included as separate files lists of all novel internal exons identified in our analyses (Additional file 2).
Artifact-containing parameters were excluded in subsequent analyses (Additional file 1: Fig. S1).
The same primers used in the qPCR with forward primer labeled with 6-FAM (6-carboxyfluorescein) were used in the T-RFLP analyses (Additional file 1: Table S1).
Thus produced epothilone glycosides and their position of glycosylation were also further confirmed by HR-QTOF ESI-MS/MS analyses (Additional file 1: Figure S3 (B-E)).
These were removed before subsequent analyses (Additional file 10).
These were excluded from all subsequent analyses (Additional file 5).
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