Heuristic maximum parsimony (MP) analysis of the dataset #1 also produced the identical tree topology as in the above analyses (figure not shown).
Maximum-likelihood (ML) analysis using dataset #1 under the transversional substitution model with gamma correction and invariable-site assumption (TVM + I + Γ) produced exactly the same tree topology as that found in the BI analyses (figure not shown).
Initial analyses with yearly average values of NO2 and analyses where NO2 was measured continuously were performed with results quite similar to the 4-weeks variable (figures not shown).
The funnel plots of stratified analyses didn't show asymmetry (figure not shown), but the results of Egger's test suggested that publication bias was evident in sub-infants studies of the GCN
In TE2 and TE13 cell lines, manual sequencing analyses were performed, but no genetic alterations of DARPP-32 were found (figure not shown).
Flow cytometric (Figure 6C) and ELISA analyses (data not shown) of A549-T cells transfected with the different shCEACAM6-plasmids confirmed the complete absence of CEACAM6 expression in the confluent growth stage, while CEACAM1 was still expressed.
The parameters that impact the state switches between "resolved" (State 0) and "self-reinforced" (State 1) of final IL-8 levels are examined through both sensitivity analyses (data not shown) and parameter scans (Figure 2B D) using Matlab Simbiology or Systems Biology Workbench.
Prior to the Bayesian analyses shown (Figure 2 and Figures S1.1 S1.33 in Supporting Information File S1), preliminary analyses (results not shown) were carried out using parsimony and distance methods for the purposes of monitoring the progress of the project and for examining partial sequence data (PAUP*4.0b10 [95] and SEQBOOT, PROTDIST, NEIGHBOR and CONSENSE in PHYLIP 3.6a3 [93.6a3
Microscopic analyses of histologic tissue sections revealed that there were no observable abnormalities in various tissues, including the brain (Figure 1E, not shown).
(Table 5; Figure 2A; Figure for PFS not shown).
