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Lungs from each mouse were used for histopathology analyses and quantification of the bacterial loads.
All analyses and quantification of vessel parameters were performed using ImageJ (v1.44 public domain software http://rsbweb.nih.gov/ij/).
Cell cycle analyses and quantification of genomic DNA fragmentation were performed using the Cell Cycle Detection Kit (KeyGEN, Nanjing, China) according to the manufacturer's protocol.
The large-scale analyses and quantification of phosphoproteins and/or phosphopeptides using mass spectrometry are known as phosphoproteomics and have been employed mostly in studies of cancer [ 2, 10] and neurodegenerative disorders studies [ 9, 15].
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Analyses and quantifications of these samples were performed using Image J software (free access).
Analyses and quantifications of these samples were performed using ImageJ software that allows counting dots on 8 bits image and the plugin 'Counter cells' allows analyzing cells number.
Thereafter, analyses and quantifications of these samples were performed using Duolink ImageTool software (developed by Olink Bioscience) Correlations between the three biomarkers were studied.
Scanning, analysing and quantification of blots were performed via the Odyssey Infrared Imaging System (LI-COR Biosciences).
Uncertainty analyses allow the identification and quantification of the factors that contribute to the potential misclassification of the ecological status of water bodies, helping to improve the sampling design used in monitoring.
Analyses of mouse sleep wakefulness and quantification of EMG were similar to that used for the rat recordings, including the use of SleepSign for Animal.
These findings were supported by BAL fluid analyses, including inflammatory cell counts and quantification of MIP-2 between GGA treated-BLM mice and vehicle treated-BLM mice.
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