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A total of 136 aligned positions were selected for the initial phylogenetic analyses (alignment available from DB upon request).
Primary data analyses, alignment and filtering utilized the Pacific Biosciences® SMRT portal.
TF carried out all of the bioinformatics analyses, alignment of clone sequences, identified early replicating islands and participated in the drafting of the manuscript.
Plasmid construction; plasmids and primers used; NMR data; vector maps; PCR analyses; alignment of FusΔeqx5 illumina reads; LC/MS and UV data; H and C spectra.
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For phylogenetic analyses, alignments were generated that included additional IR sequences (restricted to the 11 kinase sub-domains of the TK domain or to the L1, cysteine-rich and L2 sub-domains of the ligand domain).
IK took part in conception, performed sequence analyses, alignments, phylogenies, drafted and wrote the manuscript.
AB took part in conception, performed sequence analyses, alignments, phylogenies, contributed to and revised the manuscript.
For the phylogenetic analyses, alignments of the adenylation and condensation domains were were made with CLUSTALX (version 1.81).
For phylogenetic analyses, alignments included additional InR sequences delimited by the well conserved tyrosine kinase (TK) subdomain.
In order to be able to analyse alignment patterns, the notions of business architecture, application software architecture, and ICT platform architecture are introduced and defined.
The Sorting Intolerant from Tolerant (SIFT) BLink program, (http://sift.jcvi.org), [43], analyse alignments of orthologous, or paralogous sequences and predicts whether an amino acid substitution will affect protein function.
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CEO of Professional Science Editing for Scientists @ prosciediting.com