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For the ROI analyses, a minimum of 3 regions per tissue per animal were collected and the average %ID/g and standard deviation determined.
To perform repeated analyses, a minimum of 100 μL should be collected.
To include an individual participant's data set into the analyses, a minimum of 15 appropriate EEG segments per condition was required.
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Each DNA was analysed a minimum of three times.
Analysing a minimum of 20 MUPs per analysed muscle is generally accepted in the literature.
For each tissue section analysed, a minimum of 300 muscle fibers were counted.
We analysed a minimum of 20 metaphases in each sample using FISH with chr3 specific painting probe labelled with FITC (Cambio, Cambridge, UK).
Gene amplification was assessed by analysing a minimum of 20 non-overlapping nuclei, containing at least one orange and one green signal.
Quality assurance/quality control measures included a multipoint calibration curve, recalibration of each sequence of analyses (with a minimum of one blank in each batch of a maximum of 10 samples), and duplicate analyses of approximately 20% of the positive samples.
While the assessment methods used in these published studies resemble our own in that the results were derived from model-based projections informed by shorter-term clinical trial results the timeframes adopted for those analyses were a minimum of 5 years, and sometimes a lifetime horizon.
Following these exclusions, all participants included in analyses gave a minimum of 12 attended, artefact free epochs per condition.
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