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For these analyses a maximum of four joinpoint and five line segments were allowed for each long-term trend.
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Peptides were analysed a maximum of two times and were then placed on an exclusion list for 1 min.
The caecum content samples were immediately (within 30 min) placed in an anaerobe jar and kept at 4°C until analysed (a maximum of 6 hrs).
Within-herd prevalence was established by individually analysing a maximum of 50 animals from a selection of 9 pool-positive herds (4 sheep, 1 beef and 4 dairy cattle) accounting for a total of 418 animals.
All traits were scored at the Domaine de Vassal and were expressed as the mean value for five plants per accession analysed a maximum of three years following the recommended OIV descriptors as shown in Table 1[ 1[].
Based on the Joinpoint Regression Program's recommendations for the number of time points of observations in our study, our analyses allowed a maximum of five joinpoints meaning that between one and six trend segments could be in the final model depending on the number of joinpoints detected.
2. The Structure analyses indicated a maximum of three subdivisions occurring among all isolates (Additional file 1: Figure S1) with no geographical pattern evident.
Data from non-twin siblings were included in the analyses with a maximum of 2 siblings (1 brother and 1 sister) per family.
A sample of 350 will also accommodate logistic and linear regression analyses with a maximum of 35 variables entered and 19 retained, if the rules of thumb suggested by Altman apply (n/10 variables and square root of sample size respectively) [ 45].
To investigate associated changes between: child symptoms, on the one hand, and 1) child responses (i.e., child emotional, behavioral, and cognitive responses), 2) parental availability and 3) parent-child interaction on the other, we will use the stepwise procedure in multivariate regression analyses with a maximum of 4 tested predictors.
Because of variability in outcomes, meta-analyses included a maximum of 4 studies.
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