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Responses were first analysed to remove error trials (RTs less than 100 ms and RTs greater than 1000 ms).
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To further confirm the extracellular plasmid DNA presence, a putative free DNA fraction was purified from the liquid culture medium, which was previously centrifuged and filtered to remove cells, and analysed by agarose-gel electrophoresis.
Two different genomic DNA preparations for both S. coelicolor M145 and S. lividans 66 and one preparation of S. lividans TK24 were each analysed in a 'dye-balanced' experimental design (to remove any dye bias).
The cells were then washed with PBS to remove the noninternalized compound and analysed by flow cytometry.
Sequences were edited to remove vector and adaptor sequences, and analysed by BlastN and BlastX algorithms against the NCBI databases of non-redundant nucleic acids and the protein and EST accession databases http://www.ncbi.nlm.nih.gov/BLAST/[ 64].
The cells were then washed to remove the unbound dextran, fixed with 4% paraformaldehyde and analysed for macropinocytosis by confocal microscopy.
The freeze-dried samples and reference materials were analysed in triplicate (3 mg) by heating at 50 °C for 45 min initially to remove residual moisture left in the sample and this was followed by heating to 105 °C in alumina crucibles at a heating rate of 20 °C min−1 in nitrogen (BOC, UK) at a flow rate of 50 mL min−1.
In order to remove any contaminants introduced after death and to extract the collagen fraction, all analysed bone samples were treated using a protocol known as Gelatinization.
After the incubation, medium was collected and centrifuged at 14,000 g to remove cellular debris and placed onto recipient cells for different times, as indicated, or analysed for cytokines and growth factor content, as described below.
A politician to remove.
Dried samples obtained after fermentation downstream treatments were dissolved in water and analysed whereas samples obtained from shakeflask experiments were dissolved in water and diafiltered with 2 volumes of bidistilled water on 3 kDa centrifugal filter devices (YM-10 Centricon, Millipore, Bedford, MA, USA) at 5000×g to remove salts and low molecular weight contaminants.
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