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In contrast, in our study we analysed the activation of the GPHR TMDs based on the TMD_In and TMD_Ac structures of the three receptors.
We analysed the activation of cellular markers that are characteristic of apoptosis by immunoblotting.
To further verify the importance of the CWI pathway for ccw12Δ cells, we analysed the activation of Slt2p.
To assess whether any other caspases compensated for the loss of caspase-7, we analysed the activation of the effector caspase, caspase-3.
To determine if WLS also regulates endogenous WNT/β-catenin signalling, we analysed the activation of the BAR-luciferase reporter in A375 cells transfected with various siRNAs.
In order to investigate the molecular pathways responsible for the reduced muscle growth, we analysed the activation of the ubiquitin proteasome and the autophagy-lysosome systems.
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Alk4* expression and function were tested by analysing the activation of Smad2 (P-Smad2) after Dox or SB treatment in western blots.
For the analyses, the activation of the reading condition could be subtracted from the activation of the other conditions.
Analysing the activation of caspases upon stimulation with Mitoxantrone or cisplatin at concentrations and time points leading to DNA fragmentation in control cells but not in NCI H460-Akt1 cexhibitedbited a kinetic delay as well as quantitative, but not qualitative differences in the activation of the caspase cascade.
To analyse the activation of HER2 and its downstream related phosphoinositide-3 kinase/protein kinase B (PI3K/AKT) and mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK1/2) signalling cascades or to the mammalian target of rapamycin protein (mTOR) signalling pathway, we performed Western blotting and immunohistochemical analysis of each individual animal tumour.
Thus, PHB knocked-down HeLa-myc cells were infected with VSV, exposed to a pulse of VSVG traffic [ 10], and analysed for the activation of SFKs using Western blotting.
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