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In both methods the analysed surface was identified from field controlled geological maps.
The physico-chemical parameters of the analysed surface water samples of the Pratapgarh district including statistical measures such as minimum, maximum, average values and standard deviation are given in Table 2.
The TH of the analysed surface water of the study area varies between 86 and 716 mg L−1 (avg. 217 mg L−1) indicating moderately hard to very hard type of surface water.
The calcium hardness of the sampled surface water varied from 30.74 to 100.26 mg/L, with a mean of 55.15 mg/L, while magnesium hardness ranged between 21.02 and 68.31 mg/L, with a mean of 37.16 mg/L All the analysed surface water samples were below the WHO permissible limit of 200 mg/L for magnesium hardness.
The (Na+ + K+)/Cl− molar ratios in the analysed surface water from the Birim River basin were greater than 1 (> 1) (Fig. 5c), indicating that halite, as well as silicate weathering, such as potash plagioclase and sodium plagioclase, was the source Na+ and K+ ions (Lin et al. 2016a).
With the use of microelectrochemical cells, the specimen surface area in contact with the electrolyte is limited by the use of glass microcapillaries and the local impedance was defined from the ratio of the local potential to the local current restricted to the analysed surface area.
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Therefore, it can be presumed that the analysed surfaces might display a little higher surface charge density, as estimated in the analysis presented in Fig. 6.
Superimpositions revealing shape alteration of the analysed surfaces are presented in Fig. 1.
The analysed cell surface molecules were classified into different categories according to their function: adhesion molecules (CD44; CD49c; CD49e; CD49f), receptors (CD151), and other surface molecules as ectoenzyme molecules (CD26; CD26).
Cells were washed with PBS three times and analysed free surface thiol groups by flow cytometry (Beckman Coulter).
Cells were plated at a density of 1×105 cells/well and were washed with PBS and analysed for surface or intracellular cytokine expression.
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