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The remaining analysed sections of patients analysed did not show positive staining for HMGA2.
Since oocyte nuclei measured between 20 30 µm in diameter, counting every 5th section of the ovary ensured a distance of 40 µm between analysed sections, preventing multiple counts of the same ovarian follicle.
The average number of analysed sections was 18 per animal for DA, with a range of 4 up to 6 sections in single regions.
After fluorescence microscopy, the analysed sections were additionally stained with haematoxylin and eosin and microscopically analysed to obtain histological information in this part of the tissue.
The volume was calculated by using the formula: V = td ∑ S; where ∑ S is the sum of the surface areas; t is the average section thickness; and d is the distance between two consecutive analysed sections.
We analysed sections of tumour tissue from 50 patients (mean age 63 years, range 41 77 years, 27 male/23 female) that were operated on for pancreatic cancer and from 25 patients with chronic pancreatitis.
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The upper 50 cm of the analysed section show extensive human impact on the landscape during the 20th century.
The vibration data were taken over the following five chosen stations on the metro network shown in Fig. 9: Open image in new window Fig. 9 Analysed section of the metro network Pelaw Fellgate Brockley Whins East Boldon Seaburn.
The spatial distribution of the first and second saccades (black squares and white squares, respectively) is rather broad, occurring almost at any location along the analysed section of the flight trajectories (fig. 7).
Neuronal nucleus median diameter was obtained from 50 independent measurements in the central area of the striatum on each analysed section.
For each tissue section analysed, section thickness was assessed in each sampling site and guard zones of 2.5 µm were used at the top and bottom of each section.
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