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Despite the long storage time the analysed quantities should reflect the original S. aureus content as it is recorded that freezing does not affect the viability of S. aureus in milk samples [ 38].
As all analysed quantities varied <6% when >60 slices were used, the number of slices collected from HPF (64) and chemically (69) fixed beta cells was considered to be sufficient.
We analysed quantities of DNA that were representative of clinical samples, such as circulating cell free DNA, which is typically present at 10 genomic copies per mL plasma [ 37], in order to make this study relevant to the use of methylation biomarkers in non-invasive diagnosis.
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The digitised gel images were analysed using Quantity One (version 4.6.1; Bio-Rad).
For quantification, Western Blots from at least three independent experiments were analysed using "Quantity One" software from Bio-Rad (version 4.6.8).
The relative intensities of FANCD2 bands were measured with Bio-Rad's Gel Documentation System and analysed with Quantity One software.
The bands were analysed using the Quantity One™ Software (Bio-Rad, Hercules, CA, USA).
Immunoblots were digitized (GS-800 Calibrated Densitometer; Bio-Rad) and analysed with the Quantity One 4.6.3 software (Bio-Rad).
The transferred proteins were visualised with the ChemiDoc XRS system (Bio-Rad Laboratories, HerCAles, CA, USA) using an ECL detection kit (Millipore) and analysed with the Quantity One software (Bio-Rad Laboratories).
TLC plates were exposed in a Molecular Imager FX system (Bio-Rad Labs, HerCAles, CA, USA) and analysed using the Quantity One software (Bio-Rad Labs).
The results were analysed to estimate the quantity of CO2 stored as carbonates within steel slag in each case.
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