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The analysed population was very small and differences observed between responders and non-responders did not reach statistical significance.
For the same purpose additional F. ovina plants were taken from the earlier analysed population samples [4] (1 to 4 individuals with and without PgiC2 from Bornholm, Haget, Eketorp, Mosty and Dresden).
The prevalence of S. haematobium in the analysed population was 78% with a mean egg count of 28 eggs/10 ml urine (SEM = 5) with a range of 0 to 126 eggs/10 ml of urine.
The decay of linkage disequilibrium (LD) due to recombination can be used to date the age of the mutation or that of its introduction into the analysed population on a particular haplotype.
Homogeneity of the analysed population was also confirmed with a principal component analysis (results not shown).
Finally, the analysed population comprised a consecutive series of 71 patients.
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We performed the following analyses: A) To identify the SNPs separating the 14 analysed populations, we calculated a regressing tree of the SNPs regressing on the 14 population identifiers using the R library r-part (R version 3.0.1) [ 73].
The isolated spring population from Lofoten (SL2) was also in this analysis genetically the most distinct of all analysed populations, constituting a genetic group of its own ('blue'), whereas the second spring population from Lofoten (SL1) was admixed and genetically similar to the beach populations from the same area.
This might be because of there is no any sample from this population among the analysed populations or that might be extinct in the evolutionary history of subspecies populations.
Some individuals in the analysed populations had <10 twigs.
The ethnic heterogeneity of the analysed populations may be among the confounding factors.
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