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After the second stage, 58 articles remained to be analysed in the third stage.
Serum was analysed in the third generation ELISA using BIORAD and J&J USA Kits.
Total activity of ribulose-1.5-bisphosphate carboxylase/oxygenase (Rubisco) was analysed in the third or fourth youngest leaves of two or three shoots of the same age in each replicate (all leaves from the chosen shoots pooled from each replicate plant).
Once these basic issues have been addressed, the impact of generic substitution on health and economic outcomes, analysed in the third article [ 4], would be negligible and its debate pointless.
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The top five implementation challenges were then analysed in the second round.
Once the model has been designed, it can be analysed in the second phase.
The results obtained in this first part of the study showed how the starting and end composition could generate the microstructure and suggest its influence on the mechanical properties of the porcelain tile, which will be later analysed in the second part of this paper.
The first nine SNPs were analysed in the first phase of the study and the remaining 16 in the second.
A total of 58 cases were analysed in the first annual period, 52 in the second annual period, 44 in the third annual period and 79 in the fourth annual period (each with the same numbers of matched controls).
A partly redundant set of 21 SNPs that accounted for the association signals from the discovery phase was then analysed in the second Swedish case-control cohort.
CD40L cDNA was analysed in the first instance, with RT-PCR yielding a PCR product from the proband (second eldest, affected male, which was approximately 60 bp shorter than normal.
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