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GSLs were extracted from fresh collected cancer tissues, and the glycans released from the glycolipids in the form of alditols (see 'Materials and methods') were analysed in the negative ion mode using LC-ESI-MS/MS to identify P blood group-related GSLs.
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The analyses in the negative ion mode should be done in the future and, when performed in a similar setting, the results might complete the information provided by the present study.
As shown in Fig. 1, MALDI MS analyses in the negative-ion mode using 9-AA as the matrix revealed the presence of many signals including nucleotides on the liver tissue frozen section with minimal interference from the matrix, as reported by Edwards and Kennedy [ 6].
The subsequent flow cytometry analyses consisted in the negative selection of non-DC lineage markers, positive selection of HLA-DR+ DCs and, within this defined DC population, we analyzed the frequencies of single or double CD11c+ and CD123+ cells (Fig. 6a).
UVPD and NETD have been directly compared for LC MS analyses of tryptic digests in the negative mode, with the finding that either method, when combined with complementary positive mode CID data, increased sequence coverages and peptide identifications compared to CID alone.
Additional work is in progress to analyse the negative cases using exome sequencing and copy number variation analysis.
Formic acid was analysed in negative ion mode as the deprotonated molecular ion (M - 1) at m/z 45.
They thrill in the negative.
Same problem, in the negative.
He always replied in the negative.
Make choices in the negative.
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