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The experiments were run in triplicate and each sample was analysed in duplicate.
The samples were analysed in duplicate by each laboratory along with a solution of PCDD/F standards and reference sediment.
All samples were analysed in duplicate.
Five unifloral and eleven multifloral samples were collected and analysed in duplicate (see Table 4 for description).
Samples were analysed in duplicate (patient samples from BL and FU were run on the same plate).
Samples of feed ingredients, diets and fish fillets, liver and kidney were analysed in duplicate using standard methods (AOAC 1997).
Each miRNA was analysed in duplicate.
At least four independent samples of each region were analysed in duplicate TaqMan assays.
For every primer pair, four different cDNA concentrations were analysed in duplicate.
All samples were analysed in duplicate in digested and nondigested conditions.
Serums were analysed in duplicate.
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