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The ability of crizotinib to interact with, and thereby stabilize ALK in intact cells, was analysed essentially as described by Molina et al.35.35
Wing discs were analysed essentially as previously described (36).
The uptake of E. coli by neutrophils was analysed essentially as described previously [ 32, 33].
The relative DNA content was analysed essentially as described by [ 67].
Apomorphine-induced circling behaviour was analysed essentially as described for 6-OHDA lesioned rats [ 31].
Blastemas were analysed essentially as described above for luciferase activity at 96 h post-electroporation after lysis by homogenisation in 50 μl lysis buffer.
Similar(49)
Essentially, for each analysed position, the ΔCt method was used to calculate a ChIP over input ratio that was corrected by the appropriate dilution factor of each analysed fraction.
Subconfluent, adherent cells were prepared and analysed for lysine-dependent plg binding essentially as described [ 27].
The stress in a thin contracting film on a rigid substrate is analysed, and obtained as an integral of essentially the shear modulus and thickness of the curing film.
Furthermore, the combinatorial library screened is very small (256 variants), only about 20 variants were analysed and significant modulations are found in essentially all of them.
The cell culture medium was harvested after 24 h and analysed for SEAP activity according to the protocol essentially as described by the manufacturer (Clontech, Palo Alto, CA, USA) using a 96-well plate reader (Vecter 3, Perkin Elmer Life & Analytical Sciences, Boston, MA, USA) with excitation set at 360 nm and emission at 460 nm.
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