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Brain tissues can then be analysed directly using in vitro electrophysiological techniques normally only possible on animal brain tissue.
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For example, introduction of mutations or alteration of the solution conditions to specifically destabilize the native state allows transient folding intermediates to be trapped at equilibrium and directly analysed using NMR.
For determination of eGFP transgene expression, harvested eGFP mRNA-electroporated or lentivirus-transduced BMSC cultures were washed once with PBS, resuspended in PBS and directly analysed using an Epics XL-MCL analytical flow cytometer.
Digests were stopped by addition of trifluoro acetic acid (TFA) to approx. pH 3. 10% of the peptide mixture was analysed directly, the remaining 90% were enriched for phosphopeptides by the use of TiO2 as described in [ 48].
Cells infected with GFP+ viruses were washed (0.1% BSA, 0.01% azide in PBS) and analysed directly for green channel fluorescence.
Subsequently, cells were either analysed directly or maintained under standard culture conditions for 4 h.
Stable cell pools were analysed directly after outgrowth in selection medium.
Axial data can be directly analysed or used to produce multiplanar images.
When only the blood cultures containing Gram-positive bacteria were analysed, by using the McNemar test, it was again shown that the direct smear method was always significantly better compared to analysis directly from blood cultures, p ≤ 0.002.
PCR products were sequenced directly (through both strands) and analysed using the Basic Local Alignment Search Tool (BLAST) to establish that they were within the target clade.
The nanoparticle uptake was analysed quantitatively using high-content analysis.
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