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When EcoRV and XbaI were used to digest genomic DNA the blotting always resulted in a single band for all the genes we analysed (data not shown).
There were no differences at baseline between the patients who had their sample eliminated and those who were actually analysed (data not shown).
Overall, there were no statistically significant differences in change in serum concentration of any inflammatory markers during AI therapy when all cases were compared with all controls (data for all cytokines at 1 and 6 months are provided in Supplementary Tables 3 and 4) or when matched cases and controls were analysed (data not shown).
The observed gradient of PAX6 had disappeared by 9 PCW (Fig. 2D and H), although the EMX2 gradient was maintained at 9 weeks (Fig. 2C and G) and at all other stages analysed (data not shown), even though EMX2-expressing cells had predominately translocated to the CP.
On the contrary, genes included in the C1A cathepsin O, C13 GPI protein transamidase, C2 calpain, C14 caspase, C50 separase and I31 thyropin groups, did not show a specific developmental pattern, having most of them a similar level of expression in all the mite stages analysed (data not shown), with normalised expression values among 10 and 100.
Cocolonization rates were also stable over the four time periods analysed (data not shown).
Similar(21)
Cases showing absent or low β-actin signals were excluded from the analyses (data not shown).
Haplotype analysis was not consistently more informative than the individual SNP analyses (data not shown).
Efficiency of RNAi mediated Dpp knock-down was confirmed by RT-PCR analyses (data not shown).
Transcripts for all eight genes showed similar patterns by both RT-PCR and microarray analyses (data not shown).
Probe size and integrity have subsequently been confirmed via Agilent Bioanalyzer 2100 NanoChip analyses (data not shown).
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