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Real-time PCR conditions were; 95°C for 10 minutes followed by 50 cycles of 95°C for 15 sec and 60°C for 1 min. All reactions were run singleplex and analysed and quantified using the ΔΔCt method.
16-bit images were analysed and quantified using the Odyssey analysis software.
All reactions were run single-plex and analysed and quantified using the ΔΔCt method.
The relative intensities of immunoblot bands were analysed and quantified using ImageJ (National Institutes of Health) software.
The microarray spots were analysed and quantified using BlueFuse software (Version 3.1; BlueGnome Ltd ,Cambridge), which uses statistical parametrical models for accurate quantification of the spot signal and background noise.
X-ray films were analysed and quantified using a CCD camera (Fuji LAS 3000) and Aida image analyser software.
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Surface topography of wires was measured by an optical microscope and quantified using surface roughness testing.
They were scanned at two scales with two types of micro-computed tomography (micro-CT) and quantified using image analysis.
Associations between continuous measurements were illustrated using two-way scatter plots and quantified using Spearman's correlation coefficients.
Extracted DNA was amplified and quantified using qPCR on a LightCycler 480 (Roche) thermal cycler.
Volume measurements were obtained using 3D fluorescent images and quantified using Volocity software (Perkin-Elmer, Waltham, Mass).
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