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The frequent methylation of the C-P3H genes in NHL prompted us to also analyse expression of the C-P4H genes in the same panel of cell lines.
Quantitative real-time PCR (qRT-PCR was used to analyse expression of seven of the differentially expressed genes in a subset of 73 samples (for baseline characteristics of this subset, see online supplementary table S4).
Although in our investigations we did not analyse expression of the M. persicae H2 subunit in response to application of H1 dsRNA, the likelihood is that significant cross-suppression will also have occurred.
To explore the regulatory mechanism of OsNPF7.2, we analyse expression of the major regulators in cell cycle, namely, cyclin-dependent kinases (CDKs) and their regulatory partner cyclins.
Further, we analyse expression of potential targets for apple miRNAs.
Confocal imaging (Leica SP5-II LSCM) was used to analyse expression of sarcomeric proteins and to assess RYR2 subcellular distribution.
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A more recent report, analysing expression of an X-linked β-galactosidase transgene, indicated that a small percentage of cells reactivate the Xi at 11.5 dpc [12].
By immunofluorescence analyses, expression of α-dystroglycan also appeared normal in dy3K/δE3 limb, diaphragm and heart muscle and in sciatic nerve (Fig. 4A).
We then analysed expression of CEBPδ in metastatic breast cancer lesions.
However, except for PDPN determinations, we could identify only one work analysing expression of each of these markers.
To test this hypothesis, we analysed expression of hypoxia-related genes in the central and peripheral retina from 13 human donors between the age of 17 and 92 years without diagnosed retinal pathologies (Table S1).
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