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Both epifluorescence microscopy and Confocal laser scanning microscopy were used to monitor and analyse cells cultured on either tissue culture surface or 3D scaffolds after the cells were stained with CellTracker™ or polymersomes encapsuling Rhodamine B octadecyl ester perchlorate.
We used monoclonal anti-pancytokeratin antibodies C-11 to analyse cells expressing cytokeratins, markers of cells of epithelial origin.
Data analysis was performed with FlowJo Software (Tree Star, Version 8.6.6 from FlowJo Africa, http://www.flowjo.com/home/africa/) with Boolean gating to analyse cells with multiple fluorescent markers.
To analyse cells in S phase, cells were grown in overnight cultures in the appropriate drop-out SC medium containing raffinose at 30°C.
The eye lens offers the opportunity to analyse cells of different developmental stages and with different levels of transcriptional activity in a single histological section (Dahm et al. 1998; Gribbon et al. 2002).
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The Na reference electrode was also introduced to analyse cell resistances.
Here we analyse cell polarisation in the follicular epithelium of Drosophila, an epithelium that arises by a mesenchymal epithelial transition.
Hoechst 33258 staining is usually used to analyse cell nuclear morphologies after apoptosis.
At the prosecutor's office, Luisa was told that the staff there would examine footage from security cameras and analyse cell phone records for clues about her son's captors.
The chemotaxis plugin was used to analyse cell behaviour.
The MTT assay was utilized in order to analyse cell viability of keratinocytes.
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