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For each assay an identical amount of non-isotopically derivatized Val-AMP was added as an internal standard, and experiments in the presence or absence of the analog were performed using the deuterium (d15) labeled derivatizing reagent [11].
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Biochemical inhibition studies of these analogs were performed using a mammalian guanase.
SPECT/CT studies were performed using [131I]-I2-PARPi [131I]-I2-PARPi [131I]-I2-PARPimed with the structurandanalog [124I]-I2-PET/CT
Yeast dilution bioassays were performed using S. cerevisiae wild type and antioxidation mutants, sod1Δ, sod2Δ and glr1Δ, against the seven most active benzaldehyde analogs.
Preoperatively, an analog templating was performed using a transparent drawing of the prosthesis with a 20% magnification fitted on antero-posterior radiographs of both hips.
Analog hard-copy templating was performed using transparent sheets on which the contours of the prosthesis were depicted.
In view of this large installed base of analog equipment, many new installations will still be performed using new analog equipment.
The very fast angle-measurement is performed using parallel analog signal processing, 24 MHz finite state machine with dedicated peripherals and an 8 Mbit/s SPI interface.
Conversion of the analog input to digital signals was performed using a model Micro1401 interface (Cambridge Electronic Design) connected to an IBM-compatible computer.
The numerical solution of the aforementioned initial boundary value problem is performed using the analog equation method, a BEM based method, leading to a system of nonlinear differential-algebraic equations (DAE), which is solved using an efficient time discretization scheme.
Incorporation of tyrosine analogs at specific sites in proteins was performed using an engineered orthogonal tRNA-tRNA synthetase pair from an archaebacterium.
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