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For each assay an identical amount of non-isotopically derivatized Val-AMP was added as an internal standard, and experiments in the presence or absence of the analog were performed using the deuterium (d15) labeled derivatizing reagent [11].
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Biochemical inhibition studies of these analogs were performed using a mammalian guanase.
Titrations in the absence of sCD4 and in the absence PA analogs were performed as reference.
Using the method described previously in Ref. [ 21], the FGFR1 kinase inhibition assay of NDGA and its analogs were performed by Caliper Mobility Shift Assay with ATP concentration at its Km value (262 μM).
A comparable study of IHF analogs was performed varying the proline derivatives.
Before I.B.M.'s advance, while digital computing technologies were integrated onto single silicon chips, analog functions were performed by larger and more costly separate components.
The ballistics calculations in these mechanical analog computers were performed by mechanisms like differential gears, levers, and small rods riding on the surface of three-dimensional cams.
In order to show receptor mediated uptake of AEZS-125, LHRH- receptor blocking experiments with the LHRH analog, triptorelin, were performed.
In case no proteins could be identified from the first spectrum, additional database searches of the automatically generated MS/MS spectra were performed (analog: p < 0.05).
Briefly, reactions contained a ligand analog as indicated and were performed under standard conditions consisting of incubation at 22 °C in solution containing 50 mM HEPES pH 7.3.
Analog classroom time course evaluations were performed at the end of each double-blind week.
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