Exact(12)
Trained personnel at each site collected anal samples by rotating a saline water moistened nylon flocked swab in the anal canal for about 2 minutes.
Anal samples were collected as previously described [ 13].
Beta-PV DNA was detected in 116 (64.1%) out of 181 anal samples included in the study.
Anal samples for the detection of HPV will be collected by trained medical staff using Digene sampler kits at each study visit.
A higher prevalence of HR types has been found in anal samples from HIV-infected patients in previous studies, with rates of up to 70% observed [ 7].
Anal samples, collected with a Dracon swab in PreservCyt, were used both for liquid-based cytology and HPV testing by the Linear Array HPV Genotyping Test.
Similar(48)
DNA was extracted from a 200-μl aliquot of the original anal sample using an automatic DNA extractor (Biorobot M48 Robotic Workstation; Qiagen, Valencia, California, USA).
Six female and 4 male asymptomatic sexual contacts of M. genitalium infected men were tested; a throat and anal sample in 1 man and a cervical sample in 1 woman were positive for M. genitalium.
Peri-anal samples for culture were obtained using Staplex II cotton swabs (Staplex, Etobiocoke, Ontario, Canada), which are used to swab the peri-anal area in a circular motion moving from the rectum out [ 27].
UMB001 was a virulent outbreak strain isolated from the blood of a patient in the SICU; UMB002 was a strain isolated from a peri-anal sample that was not part of the outbreak but was isolated at the same time in the MICU; UMB003 was also present at the time of the outbreak, but was isolated from a wound sample and was not related to the outbreak strain.
Thus, considering the similar genome features between MojV and other henipaviruses, we confirmed that MojV could be classified as a new species closely related to Henipavirus spp. Specific nested primer sets targeting the L gene of MojV were designed to separately re-evaluate the 34 anal swab samples and some tissue samples.
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