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The skin on the birds' head was anaesthetized using a surface anaesthetic (Xylocain; Astra Zeneca, Wedel, Germany) and incised dorsally.
Animals were anaesthetized using xylazine and ketamine anaesthetic combination.
On day 64, all fish were collected and anaesthetized using benzocaine.
48 h post infection, fish was anaesthetized using ms-222 and euthanized by decapitation.
Firstly, animals were anaesthetized using chloroform, then weighted and sacrificed at different time intervals.
Day 0: Rats were anaesthetized using isoflurane (Forene®, Abbott, Germany)/nitrous oxide inhalation.
Second, the sedation was titrated to Ramsay 3 4 points in the prior study, and the rabbits were anaesthetized using ketamine with midazolam.
Mice were anaesthetized using Chloral hydrate and placed into a stereotactic frame with nose and ear bars specially adapted for mice.
At the final day of treatment, rats were anaesthetized using 2.5%% isoflurane in oxygen (1 L/min) and sacrificed by decapitation.
Sixty minutes after administration of the drug/vehicle, the animals were anaesthetized using anesthetic ether and a midline incision was made just below the xiphoid process.
The mice were anaesthetized using an intraperitoneal injection of 7.5 μl per gram body weight of KMA mix (25.5 mg/ml ketamine, 40 μg/ml medetomidine, 0.1 mg/ml atropine in saline).
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