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a. Include an unstained control for gating.
i. Include an unstained control for gating.
Include an unstained control for gating.
Values were expressed as mean fluorescence intensity ratio compared with an unstained control (fold change).
Aliquots of 1 ml bacterial suspension were added into flow cytometry tubes for staining treatments with a depolarized control and an unstained control.
An unstained control was also tested for each sample, as well as positive controls for each of the seven antibodies from one of the samples in each batch.
Similar(54)
Images were thresholded using a predetermined global threshold based on unstained control samples (in this process pixels with a value greater than the threshold value were assigned as 'object' pixels while those with values smaller than the threshold value as dark or subthreshold pixels).
Unstained control cells and a single stain control for each fluorochrome were prepared and used to set up flow cytometric compensation.
We ran an unstained (autofluorescence control) and permeabilized PBMC sample.
Unstained control is delineated by a gray line.
However, it can be seen in Figure 3 that the population of CD90+CD45RA− cells stained with anti-HLA-DR antibody showed an increased Alexa 700 fluorescence compared with the Alexa 700 unstained control; essentially the entire population shifted.
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