Exact(4)
Regarding the ure2 cluster, ureF2 (BOV_1316) and ureT (BOV_1319) are pseudogenes, while the remaining genes are conserved, suggesting that this cluster could encode an unknown activity.
Because TERT mutants bearing deletions in their TR-binding domain functionally rescue both the circulating blood cells and defective differentiation of hematopoietic cells in zTERT morphants, an unknown activity of the RT domain of TERT, other than telomerase activity, may be crucial for hematopoiesis.
For example, the reaction (XN000004663) "NO --/ Caspase-1(h)" means that NO semantically inhibits an unknown activity of Caspase-1.
Previous data obtained in our laboratories had disclosed for the first time an unknown activity of 𝒟lentiginosine: its capacity to induce cell death in both lymphoid and epithelial cells through caspase activation.
Similar(56)
All other combinations (including those made of a low-activity haplotype and an unknown-activity haplotype or a newly described haplotype) were parsimoniously pooled together in a "fast/unknown" phenotype category, thus ensuring conservative estimation of slow acetylation prevalence in each population sample.
Upon characterization of the expressed enzyme, however, we discovered that it acts on mevalonate to generate mevalonate 3-phosphate, a previously unknown activity (see Figure 1).
We added protein biochemistry to our mainstay genetics and discovered a previously unknown activity we termed 'E4', which acts on mono-ubiquitylated proteins and promotes poly-ubiquitin chain elongation in conjunction with E1 E3 (Koegl et al, 1999).
Although the wild-type has an unknown esterase activity to MeOAc, the activity was not high enough to grow from 270 mM MeOAc as sole carbon source, reaching OD600 of 5.28 ± 0.2 in 32 h.
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This cluster also includes FADS3, a gene that shares 52%and62%2% sequence identity with the FADS1 and FADS2 genes, respectively, and encodes a desaturase of unknown activity.
Implementation of a Tanimoto coefficient cutoff of 99% for similarity between known actives from the high-throughput screen and compounds with unknown activity yielded a total of 1204 novel hits including 849 benzamides, 91 benzoxazepines, and two phenylethynyl-phenyls.
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