Andrew Simms of the New Economics Foundation said: "We face a unique alignment of economic and environmental interests.
Average percent identity was approximately 92%, with an average alignment length of 384 bp, and 51,087 contigs (91%) produced a unique alignment to a single chromosome (Table S6).
Additional sequence was able to improve the rate of alignment in maize, but in rice, where shorter sequences were more likely to be suitable for a unique alignment, additional sequence just increased the likelihood of sequencing errors reducing the alignment quality.
This unique alignment of technology offers an enormous reduction in the number of fabrication steps when compared with more traditional methods of manufacture.
Owing to the unique alignment of the amide groups in an α-sheet, we expect to see strong sequential dNN Nuclear Overhauser Effect NOEE) crosspeaks along the backbone since the NH groups are aligned on one side of the chain instead of alternating between opposite faces as in β-sheet structure.
Subsequent filtering steps removed any primers that violated the following constraints: maximum of 50 bp distance between the mutation and one of the primers in a pair, unique gapless alignment of both primers in a pair (as single reads) with tolerance of up to one mismatch, unique alignment of primer pairs via paired alignment with Bowtie 0.12.7 [ 19].
Direct alignment of the 35 bp Illumina reads resulted in the unique alignment of approximately one-third of the sequences.
For instance, features such as the presence of repetitive DNA make the unique alignment of sequence reads difficult or impossible.
Because pre-EnsEMBL Sus scrofa build 7 [ 14] comprises approximately 70% of the porcine genome, unique alignment of reads does not guarantee that there is no other similar (duplicated) sequence in the remainder of the genome.
It has been proposed that this specificity is due to the unique alignment of the monoalkylated guanine in the minor groove, and is not affected by the surrounding nucleotide context (Sastry et al. 1995; Gargiulo et al. 1995).
Unique alignments of identical sequences with fewer than five reads and more than 200 reads were discarded.
