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The dataset was then reduced to ten sequences using an overlapping fragment of 120 bp from position 16042 to 16161.
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The optimal split position of an overlapping fragment pair is that which minimizes the sum of the contributions from the updated genomic distance and the number of mismatches retained in the updated fragments.
Each 3D structure of ℓ residues is encoded into a linear sequence of HMM-27 structural letters and results in a sequence of ℓ - 3 structural letters since each overlapping fragment of four consecutive residues corresponds to one structural letter.
After removing all chains with less than 40 residues and the few with unknown sequence, each possible overlapping fragment of length k was extracted.
Therefore, we can conclude that the regulatory activity of AF3 in this assay is located in AF3.6, and that additional activity may be present in the overlapping fragment of AF3.5 and AF3.6.
The libraries included paired-end (PE) (insert size: 500 bp) and overlapping fragment (OF) (insert size: 180 bp) libraries for the HiSeq 1000 sequencer, and single-end (SE) and PE libraries (insert size: 4 kb) for the GS FLX+ system.
To implement this comparison, ustacks breaks the sequence of each stack into a set of overlapping fragments of equal length k (k-mers) (Edgar 2004; Vinga and Almeida 2003).
Re-isolation of the plasmids and sequencing of the inserted DNA sequences showed that both plasmids carried overlapping fragments of a gene from S. stipitis encoding a putative sugar transporter [EMBL: ABN64726].
In this study, multiple primers were designed to amplify overlapping fragments of a gene so that the entire gene could be screened by TILLING.
To measure recombination rates, we analysed the restoration of a functional hyg gene that confers Hyg-R by a recombination event between the two overlapping fragments of the hyg gene.
A multiplex PCR approach was used to amplify overlapping fragments of the complete mitochondrial control region (CR) and cytochrome c oxidase subunit I (COI) gene, a separate PCR amplified a fragment from the nuclear melanocortin 1 receptor (MC1R) gene (7×214 bp avg., 10×209 bp avg. and 1×95 bp amplicons respectively).
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